Utica University's St. Petersburg campus on 9400 4th Street North is a dynamic hub dedicated to nursing education, offering accelerated and hybrid nursing programs tailored to effectively meet Florida's increasing demand for healthcare professionals. The campus features programs such as the Accelerated Bachelor of Science in Nursing (ABSN) and the Direct-Entry Master of Science in Nursing (DEMSN), both structured to provide a comprehensive educational experience through a blend of online coursework, hands-on labs, and clinical rotations in the Tampa Bay area. These programs are especially designed for individuals seeking rapid and efficient entry into the nursing field.

The state-of-the-art training facility at this campus is equipped with modern classrooms, computer labs, and a clinical laboratory, ensuring students receive a well-rounded, practical education. A highlight of this campus is its strong sense of community involvement, with multiple outreach programs enhancing student engagement and service. Graduates from here are well-prepared to take the NCLEX-RN exam and have successfully found employment in major hospitals across Florida, evidencing the program's solid reputation and accreditation by the Commission on Collegiate Nursing Education (CCNE). Despite the discontinuation of the skills lab, the St. Petersburg campus continues to uphold its commitment to producing competent, practice-ready nurses who can seamlessly transition into professional healthcare settings.